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Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Brand: Thermo Scientific™ 29986
This item is not returnable.
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Description
Thermo Scientific™ EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form covalent bonds.
Highlights:
- Water-soluble, polyethylene glycol (PEG) spacer arm
- Much higher coupling yield than achieved with photoactivatable biotin
- Psoralen moiety reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
- Covalent attachment occurs with exposure to UV light (approximately equal to 350nm)
- Great for biotinylating DNA/RNA probes (∽1 biotin/10-20 bases)
- Ultrahigh sensitivity relative to radiolabeled probes (<100 femtograms)
- Does not interfere with hybridization
Specifications
| Psoralen-PEG3-Biotin | |
| Store at 4°C protected from light and moisture. Shipped at ambient temperature. | |
| Cell-Impermeant | |
| Psoralen | |
| Biotin | |
| Biotin & Analogs |
| 5 mg | |
| EZ-Link™ | |
| Light-activated | |
| DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water | |
| Extra-long, Pegylated |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- EZ-Link Psoralen-PEG3-Biotin
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : EZ-Link Psoralen-PEG3-Biotin
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Product Title
Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin > 5mg
For Research Use Only. Not for use in diagnostic procedures.
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