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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit
Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Brand: Thermo Scientific™ 21425
Description
Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.
Highlights:
- Shorter spacer arm than Sulfo-NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
- Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
- Water solubility eliminates the need for harmful organic solvents
Specifications
EZ-Link Sulfo-NHS-Biotinylation Kit | |
10 reactions | |
1 to 10 mg | |
Water | |
Antibodies, Proteins |
Sulfo-NHS-Biotinylation Kit | |
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody • EZ-Link Sulfo-NHS-Biotin, 25 mg • PBS Pack (makes 500mL), 1 pack • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns • HABA Solution, 1 mL • Avidin, 10 mg Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C. |
|
EZ-Link™ | |
Biotin |
Safety and Handling
- EZ-Link Sulfo-NHS-Biotinylation Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P311-Call a POISON CENTER/doctor/ .
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulfo-NHS-Biotin, Sodium chloride, Sodium phosphate dibasic
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For Research Use Only. Not for use in diagnostic procedures.