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Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Brand: Thermo Scientific™ R1101
109.20 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Description
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension
Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)
Specifications
Aminoallyl-dUTP Solution, 50 mM (50 μL) Store at –20°C. |
|
DNA Polymerase | |
Aminoallyl | |
Dry Ice | |
50 mM |
Liquid | |
Direct Labeling | |
50 μL | |
Standard PCR, Gene Expression, Non-Radioactive Labeling |
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For Research Use Only. Not for use in diagnostic procedures.