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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
193.00€ - 740.00€
Specifications
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|---|---|
| Format | Stand-alone Enzyme |
| Reaction Speed | 60 min. |
| Technique | Reverse Transcription |
| Optimal Reaction Temperature | 42°C to 45°C |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10121360
|
Thermo Scientific™
EP0451 |
10,000 Units |
193.00€
10000 units |
In Stock
Log in to see stock. 
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11541515
|
Thermo Scientific™
EP0452 |
5 x 10,000 Units |
740.00€
Pack of 5 |
In Stock
Log in to see stock.
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Description
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).
Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Specifications
• RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|
| 60 min. | |
| 42°C to 45°C | |
| Reduced | |
| 200 U/μL | |
| Separate components | |
| Up to 13 kb |
| Stand-alone Enzyme | |
| Reverse Transcription | |
| RevertAid H Minus | |
| Dry Ice | |
| First-Strand cDNA | |
| Reverse Transcription | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.