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Thermo Scientific™ T4 RNA Ligase (10 U/μL)
Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.
Brand: Thermo Scientific™ EL0021
55.12 EUR valid until 2024-09-27
Use promo code "21650" to get your promotional price.
Description
T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.
The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.
- Source: E. coli cells with a cloned gene 63 of bacteriophage T4
- Molecular Weight: 43.6 kDa monomer
- The absence of ribonucleases, exodeoxyribonucleases, endodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
- E.coli cells with a cloned gene 63 of bacteriophage T4
Molecular Weight:
- 43.6kDa monomer
Definition of Activity Unit:
- One unit of the enzyme catalyzes the conversion of 1nmol of 5ft.-[32P]-(A)12-18 to a phosphatase-resistant form in 30 min. at 37°C
- Enzyme activity is assayed in the following mixture: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 10mM DTT, 1mM ATP, 10μM 5'-[32P]-(A)12-18 (10μM in 5ft.-termini)
Storage Buffer:
- The enzyme is supplied in:20mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA, 0.03% (v/v) ELUGENT Detergent and 50% (v/v) glycerol
10X Reaction Buffer:
- 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 100mM DTT, 10 mM ATP
Inhibition and Inactivation:
- Inhibitors: metal chelators, SH group-modifying reagents (8).
- Inactivated by heating at 70°C for 10min.
Recommended for:
RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate (1); Joining RNA to RNA (2); Synthesis of oligoribonucleotides and oligodeoxyribonucleotides (3, 4); Specific modifications of tRNAs (5); Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5ft. RACE (Rapid Amplification of cDNA Ends) (6); Site-specific generation of composite primers for PCR (7)
Note:
The recommended BSA concentration in the reaction mixture is 0.1mg/ml.
Specifications
10 U/μL | |
10X Reaction Buffer | |
T4 RNA Ligase |
T4 RNA Ligase | |
1000 U |
For Research Use Only. Not for use in diagnostic procedures.