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Gibco™ Advanced MEM
Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Specifications
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
|---|---|
| Classification | Animal Origin |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | MEM (Minimum Essential Medium) |
Requires: Supplementation
Description
Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
This Advanced MEM is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Gibco™ Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.
iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco Advanced MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Specifications
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| 1 X | |
| MEM (Minimum Essential Medium) | |
| Sterile-filtered | |
| Low Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA) | |
| cGMP-compliant under the ISO 13485 standard | |
| Ambient |
| Animal Origin | |
| Liquid | |
| Reduced Serum | |
| Sterile-filtered | |
| No Glutamine, No HEPES | |
| Gibco™ | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Safety and Handling
missing translation for 'shelfLife' : 12 Months From Date of Manufacture
For Research Use Only. Not for use in diagnostic procedures.
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Product Title
Gibco™ Advanced MEM