Applied Biosystems™ High Resolution Melt (HRM) Software v2.0

Description

The 7500 Fast Real-Time PCR System delivers precise results with fast thermal cycling in a standard 96-well format. Achieve high-throughput HRM analysis with the 384-well 7900HT, the gold standard high throughput system.

High Resolution Melt (HRM) analysis is an alternative to dHPLC sequencing screening of new gene variants. The AB HRM application does not require temperature shifting, which results in a greater likelihood of identifying new homozygous mutations than methods that require temperature shifting.

• Shorten analysis time by auto-calling genotypes and automatically omitting the no template controls
• Minimize subject analysis by automatically grouping unknown variant clusters
• Enable easy data review with customizable plot views, expandable windows, and one-click color assignment to highlight curves of interest.
• Ability to analyze multiple targets (assays) on one plate

Identify more new variants, quickly and accurately
The ability to easily identify new variants is key for successful HRM applications. By eliminating the temperature shift step, the Applied Biosystems HRM solution was able to clearly distinguish homozygous variant samples from homozygous wildtype samples in 97.5% of the population, whereas the other HRM system from Competitor R was only able to distinguish them in 10% of the population. All genotypes were auto-called by the respective software packages and were not altered by the operator. Class 1 SNP (A/G), multiple technical replicates of nine DNA samples representing three genotypes: homozygous wildtype (G/G), homozygous mutant (A/A) and heterozygous (A/G).