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Cytiva HiTrap™ IMAC Columns
For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion
Brand: Cytiva 17-0920-03
Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Description
- Conveniently charge with your metal of choice
- Can be used with a syringe, peristaltic pump, or liquid chromatography system
- Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
- High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
- Stable in the presence of all commonly used buffers
- Available in 1mL and 5mL sizes
Specifications
| 1 mL | |
| <4 mL/min. | |
| IMAC Sepharose | |
| Polypropylene | |
| 3 to 12 | |
| HiTrap IMAC HP Column | |
| 5 x 1 mL |
| 0.27 in. | |
| 7 mm | |
| Syringe, peristaltic pump or liquid chromato graphy system | |
| 42 psi | |
| 34 μm | |
| 4°C to 30°C | |
| For optimizing purification of His-tagged Proteins |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- HiTrap IMAC HP, 1 ml, 5 x 1 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.