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Cytiva HiTrap™ IMAC Columns

For optimizing purification of histidine-tagged proteins when Ni⁺² is not the best choice of metal ion

Brand: Cytiva 17-0921-02

Product Code. 10697275

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Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

This item is not returnable. View return policy

Description

Conveniently charge with your metal of choice
Can be used with a syringe, peristaltic pump, or liquid chromatography system
Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
Stable in the presence of all commonly used buffers
Available in 1mL and 5mL sizes

Specifications

Bed Volume	1 mL
Flow Rate	<4 mL/min.
Kit Contents	Prepacked with IMAC Sepharose 6 Fast flow media
Material	Polypropylene
pH Range	3 to 12
Product Type	HiTrap IMAC Fast Flow Column
Quantity	5 x 1 mL

Diameter (English) Inner	0.27 in.
Diameter (Metric) Inner	7 mm
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system
Max. Pressure	42 psi
Particle Size	90 μm
Content And Storage	4°C to 30°C
For Use With (Application)	For optimizing purification of His-tagged Proteins

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

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Hazard Category

Hazard Statement

Precautionary Statement

P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P235-Keep cool.
P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

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