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Cytiva HiTrap™ IMAC Columns
For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion
Brand: Cytiva 17-0921-04
Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Description
- Conveniently charge with your metal of choice
- Can be used with a syringe, peristaltic pump, or liquid chromatography system
- Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
- High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
- Stable in the presence of all commonly used buffers
- Available in 1mL and 5mL sizes
Specifications
| 5 mL | |
| <4 mL/min. | |
| Prepacked with IMAC Sepharose 6 Fast flow media | |
| Polypropylene | |
| 3 to 12 | |
| HiTrap IMAC Fast Flow Column | |
| 5 x 5 mL |
| 0.62 in. | |
| 16 mm | |
| Syringe, peristaltic pump or liquid chromato graphy system | |
| 42 psi | |
| 90 μm | |
| 4°C to 30°C | |
| For optimizing purification of His-tagged Proteins |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiTrap IMAC FF, 5 ml, 5 x 5 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol