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Cytiva HiTrap™ rProtein A FF Columns
Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.
Brand: Cytiva 17-5080-01
This item is not returnable.
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Description
- Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
- Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
- Average particle size: 90μm
- Designed for use with a syringe, a peristaltic pump, or a chromatography system
- Available as 1mL and 5mL columns
- Ligand: Recombinant protein A (E.coli)
- Matrix: Highly cross-linked 4% agarose
- Binding capacity: ∽50mg human IgG/mL
- pH stability working range: 3 to 10
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Order Info
1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.
Specifications
| ≈50 mg Human IgG/mL | |
| Chromatography System, Manual | |
| 4°C to 30°C, 20% Ethanol |
| <20 mL/min. | |
| For syringe, peristaltic pump or a chromatography system | |
| 1 x 5 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiTrap rProtein A FF 5 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : Ethanol