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Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
219.00€ - 751.00€
Specifications
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
|---|---|
| Storage Requirements | 4°C to 8°C |
| Product Type | HiTrap Talon crude prepacked Column |
| Flow Rate | 1 to 4 mL/min |
| Particle Size | 60 to 160 μm |
Description
Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
- Prepacked with cobalt-based chromatography media
- Binding capacity approximately 7mg MBP
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL columns
Media
- Ligand: TALON™ Superflow Cobalt-IMAC
- Matrix: 6% cross-linked agarose
- Average particle size: 60 to 160μm
- pH stability working range: 3 to 12
1mL columns
- Column I.D.: 7mm
- Flow velocity: 1 to 4mL/min.
5mL columns
- Column I.D.: 16
- Flow velocity: 5 to 20mL/min.
Specifications
| For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH | |
| HiTrap Talon crude prepacked Column | |
| 60 to 160 μm | |
| 3 bar | |
| 3 to 12 | |
| Talon Superflow Cobalt-IMAC |
| 4°C to 8°C | |
| 1 to 4 mL/min | |
| Polypropylene | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 2
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H226-Flammable liquid and vapour.
- EUH208-Contains . May produce an allergic reaction.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, methanol, cobalt