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Proteintech™ Human CCL5 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human CCL5
Brand: Proteintech™ KE00093-96T
Description
- Recognizes human CCL5
- CCL5 (RANTES) belongs to CC chemokine family
- Strongly expressed in human and murine liver upon injury
- Produced by cell types: platelets, macrophates, endothelial and stellate cells
- CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells and basophils to sites of inflammation by interacting with G-protein-couples receptors (CCR1, CCR3 and CCR5)
- CCRS5: promote efficient anti-tumor immune responses and associated with cancer progression and metastasis
Process:
- Antibody specific for human CCL5 pre-coated onto microwells
- Human CCL5 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for human CCL5 is added to detect captured human CCL5 protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
NP_002976 | |
0.3 pg/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Human | |
Human | |
<10% | |
Blue ice | |
4.5 hr. |
3.9 to 250 pg/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
6352 | |
Solid | |
<10% | |
-20°C to +4°C | |
P13501 |
Safety and Handling
- 96TEST Human IL-3 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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