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Proteintech™ Human CCL5 ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human CCL5

Brand:  Proteintech™ KE00093-96T


Product Code. 16841506

  • 269.00€ / 96 tests

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Description

Description

  • Recognizes human CCL5
  • CCL5 (RANTES) belongs to CC chemokine family
    • Strongly expressed in human and murine liver upon injury
    • Produced by cell types: platelets, macrophates, endothelial and stellate cells
    • CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells and basophils to sites of inflammation by interacting with G-protein-couples receptors (CCR1, CCR3 and CCR5)
      • CCRS5: promote efficient anti-tumor immune responses and associated with cancer progression and metastasis

Process:

  • Antibody specific for human CCL5 pre-coated onto microwells
  • Human CCL5 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human CCL5 is added to detect captured human CCL5 protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_002976
0.3 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
4.5 hr.
3.9 to 250 pg/mL
ISO 13485
Coated ELISA Kit
DAB
6352
Solid
<10%
-20°C to +4°C
P13501
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