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Proteintech™ Human HSP70 ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous HSP70

Brand:  Proteintech™ KE00059-96T


Product Code. 16851476

  • 269.00€ / 96 tests

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Description

Description

  • Recognizes human HSP70
  • HSP70 (heat-shock protein 70) is a stress-inducible member of proteins which are high conserved chaperons implicated in protein folding, refolding, transport and targeting
    • Expression of protein can be highly activated by stressful stimuli
    • Significant up-regulation of HSP70 found in tumpors
    • Increased serum levels associated with progression of multiple diseases (atherosclerosis, heart failure, diabetes)

Process:

  • Antibody specific for HSP70 pre-coated onto microwells
  • HSP70 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for HSP70 is added to detect captured HSP70 protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_005336
1 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
3.5 hr.
31.25 to 2000 pg/mL
ISO 13485
Coated ELISA Kit
DAB
3303
Solid
<10%
-20°C to +4°C
P08107
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