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Proteintech™ Human JNK ELISA Kit

Solid phase sandwich ELISA for the quantitative determination of JNK in samples of human and mouse origin

Brand:  Proteintech™ KE00072-96T


Product Code. 16861486

  • 269.00€ / 96 tests

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Description

Description

  • For testing cell lysates
  • JNK (MAPK8, Mitogen-activated protein kinase 8, PRKM8, SAPK1, SAPK1C) belongs to MAP kinase subfamily
    • Activated by dual phosphorylation at Thr-Pro-Tyr motif during response to UV light
    • Functions as phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within transactivation domain
    • JNK activity abnormally elevated in obesity
    • Absence of JNK results in decreased adiposity, significantly improved insulin sensitivity and enhanced insulin receptor signaling capacity in two models of mouse obesity (ob/ob)
    • Mediator of obesity and insulin resistance and a potential target for therapeutics

Process:

  • Antibody specific for JNK pre-coated onto microwells
  • JNK protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for JNK is added to detect captured JNK protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_001265476
0.1 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
3.5 hr.
1.56 to 100 ng/mL
ISO 13485
Coated ELISA Kit
DAB
5599
Solid
<10%
-20°C to +4°C
P45983
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