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Proteintech™ Human JNK ELISA Kit
Solid phase sandwich ELISA for the quantitative determination of JNK in samples of human and mouse origin
Brand: Proteintech™ KE00072-96T
This item is not returnable.
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Description
- For testing cell lysates
- JNK (MAPK8, Mitogen-activated protein kinase 8, PRKM8, SAPK1, SAPK1C) belongs to MAP kinase subfamily
- Activated by dual phosphorylation at Thr-Pro-Tyr motif during response to UV light
- Functions as phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within transactivation domain
- JNK activity abnormally elevated in obesity
- Absence of JNK results in decreased adiposity, significantly improved insulin sensitivity and enhanced insulin receptor signaling capacity in two models of mouse obesity (ob/ob)
- Mediator of obesity and insulin resistance and a potential target for therapeutics
Process:
- Antibody specific for JNK pre-coated onto microwells
- JNK protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody specific for JNK is added to detect captured JNK protein
- For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| NP_001265476 | |
| 0.1 ng/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 3.5 hr. |
| 1.56 to 100 ng/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 5599 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P45983 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human JNK ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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