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Proteintech™ Human Leptin ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human leptin

Brand:  Proteintech™ KE00095-96T


Product Code. 16851506

  • 269.00€ / 96 tests

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Description

Description

  • Recognizes human leptin
  • Leptin is 16 kDa adipocyte-derived hormone, has attracted interest in field of obesity research due to role in regulation of energy balance
    • Secreted from adipose tissues in proportion to fat store, acts in hypothalamus to regulate feeding behavior
    • Plays role in tumorigenesis, tumor progression, metastasis due to oncogenic, mitogenic, pro-inflammatory and pro-angiogenic actions
    • Mutations in gene and/or regulatory regions cause severe obesity and morbid obesity with hypogonadism
    • Linked to Type 2 diabetes mellitus development

Process:

  • Antibody specific for human leptin pre-coated onto microwells
  • Human leptin protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human leptin is added to detect captured human leptin protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) regeant
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_000221
2 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
4.5 hr.
15.6 to 500 pg/mL
ISO 13485
Coated ELISA Kit
DAB
3952
Solid
<10%
-20°C to +4°C
P41159
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