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Proteintech™ Human MMP-9 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous MMP9
Brand: Proteintech™ KE00164-96T
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Description
- Recognizes human MMP-9
- MMP-9 (Matrix metallopeptidase 9, Gelatinase B) is a member of matrix metalloproteinase family
- MMP family of enzymes comprised of critically important extracellular matrix remodeling proteases whose activity implicated in normal embryogenesis, tissue remodelling and diseases (arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration)
- Proteases represent important therapeutic and diagnostic targets for treatment and detection of human cancers
- Produced by variety of normal and transformed cells (neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts)
- Destroys collagen (Type IV) in vascular basal membrane in vicinity of tumor cells which invade surrounding tissues and contribute to metastasis
- Circulating levels increased in many inflammatory disorders (atherosclerosis, hepatitis C virus infection, colorectal cancer)
- MMP family of enzymes comprised of critically important extracellular matrix remodeling proteases whose activity implicated in normal embryogenesis, tissue remodelling and diseases (arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration)
Process:
- Antibody specific for MMP-9 pre-coated onto microwells
- MMP-9 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for human MMP-9 is added to detect captured human MMP-9 protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| BC006093 | |
| 10 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 62.5 to 4000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 4318 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P14780 |
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Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Mouse Erythropoietin ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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