Cytiva PlasmidPrep Midi Flow Kit

Description

illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume. illustra plasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50mL for high copy number plasmid DNA and up to 500mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA. The kit contains fast-flow plasmid 250 columns, resuspension, lysis, neutralisation, wash, elution, and TE buffers, and a full protocol booklet with a detachable, quick reference protocol card.

• High column capacity allows for a proportional yield for input sample volume of 25 to 50mL (for high copy number plasmid) and up to 500mL for low copy number plasmid.
• Provides plasmid DNA with excellent purity and low endotoxin levels.
• Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
• Modified alkaline lysis procedure.
• Anion exchange chromatography.
• Purification format is single-use, gravity flow illustra Fast-Flow plasmid 250 columns.
• Greater than 70% (strain-dependent) of supercoiled pDNA.
• Less than10 endotoxin units (EU) per ?g of plasmid DNA.
• Kit consistently produces high yields of pure plasmid DNA with low levels of endotoxin contamination.