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Cytiva IMAC Sepharose™ High Performance Media
For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion
Brand: Cytiva 17-0920-07
This item is not returnable.
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Description
- 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
- Charge with transition metal of choice for optimized selectivity
- Small bead size for high performance purification
- High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
- Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
- Stable in pH range 3-12 (long term), and in all commonly used buffers
Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.
Specifications
| <300 cm/hr. | |
| 34 μm | |
| 4°C to 30°C | |
| Purification of Polyhistidine tagged Proteins |
| 3 to 12 | |
| IMAC Sepharose HP | |
| 100 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- IMAC sepharose high perfomance
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol