Thermo Scientific™ IMAGEN™ Adenovirus Kit using Direct Immunofluorescence Assay

Description

Rapidly detect adenovirus antigen in clinical samples and confirm adenovirus in cell cultures with Thermo Scientific™ IMAGEN™ Adenovirus Kit. This direct immunofluorescence assay utilizes a genus-specific monoclonal antibody to detect an epitope of adenovirus hexon proteins, which is expressed in all known human adenovirus serotypes¹.

Human adenoviruses are associated with a wide range of clinical diseases in both immunocompetent and immunocompromised individuals, which include infections of the respiratory tract, conjunctiva and gastrointestinal tract^2,3. Infections are common in children and can occur sporadically or in outbreaks.

Use IMAGEN Adenovirus Kit as a rapid, sensitive and specific method for direct detection and identification of human adenovirus serotypes in clinical specimens or cell cultures.

• Rapid results—within little over 30 minutes of receiving test sample or culture
• Simple—ready-to-use reagents plus all IMAGEN assays follow the same basic methodology
• Excellent sensitivity and specificity—high quality, well characterized antibodies used in the assay provide excellent sensitivity and specificity
• Easy-to-read results
• Long shelf life—24 months

Adenoviruses are non-enveloped DNA viruses of icosahedral symmetry. The family Adenoviridae comprises two genera, mammalian adenoviruses (Mastadenoviruses) and avian adenoviruses (Aviadenoviruses)⁴. At least 47 known serotypes of human adenovirus have been identified and characterized by hemagglutination, neutralization, DNA-hybridization and restriction endonuclease analysis of adenoviral DNA^2,4,5.

The laboratory diagnosis of adenovirus infection plays an important role in patient management and enables effective management and control of outbreaks. A range of techniques have been used to confirm the identification of adenovirus isolates, including neutralization tests, radioimmunoassay, DNA hybridization, electron microscopy and DNA electrophoretyping^6,7,8,9,10. These techniques can be complex, laborious and often inappropriate for routine use. Recently indirect immunofluorescence tests or enzyme immunoassays (e.g., IDEIA™ PCE Chlamydia Kit) using genus-specific monoclonal or polyclonal antibodies have been described for the direct detection of adenovirus in clinical specimens or cell culture monolayers^10,11.

Direct immunofluorescence tests utilizing specific monoclonal antibodies offer a rapid, sensitive and specific method for the direct detection of adenoviruses in clinical specimens such as nasopharyngeal aspirates and conjunctival smears or for the confirmation of adenovirus isolates in cell culture monolayers. IMAGEN Adenovirus Kit is a direct immunofluorescence test for the detection and identification of human adenovirus serotypes in clinical specimens or cell cultures.

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