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Invitrogen™ pTrcHis2 A, B, & C Bacterial Expression Vectors
Offer enhanced translation initiation and high-level expression in E. coli
Brand: Invitrogen™ V36520
Includes: All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided.
Description
- These vectors feature high-level regulated transcription from the trc promoter
- Enhanced translation efficiency of eukaryotic genes in E. coli
- The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
- This particular vector offers C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
- C-terminal Xpress epitope for easy detection with an anti-Xpress antibody
- Enterokinase cleavage site for removal of fusion tag
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| Trc, lacO | |
| None | |
| Ampicillin (AmpR) | |
| Thioredoxin | |
| 20 μg |
| IPTG | |
| Bacterial Expression Vector | |
| All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored. | |
| EK (Enterokinase) Recognition Site | |
| Restriction Enzyme/MCS | |
| pTrc |
For Research Use Only. Not for use in diagnostic procedures.