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Thermo Scientific™ Klenow Fragment, LC (2 U/μL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Brand:  Thermo Scientific™ EP0054

Product Code. 10450440

  • 88.75€ / 300 units

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Description

Description

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA
TRUSTED_SUSTAINABILITY
Specifications

Specifications

2 U/μL
DNA Polymerase I
300 units
Klenow Fragment
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2841136 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0054
Lot Number2860393 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0054
Lot Number2874287 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0054
Lot Number2915979 Certificate TypeCertificate of Analysis Date27/01/2025 Catalog NumberEP0054
Lot Number2958264 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog NumberEP0054
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Thermo Scientific™ Klenow Fragment, LC (2 U/μL) > 2μ/μL, LC, 300U

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