Thermo Scientific™ tRNA for LightShift™ Chemiluminescent RNA EMSA Kit

Description

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional ³²P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Highlights:

• Sensitive – chemiluminescent detection comparable to radioactive detection

• Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

• Flexible – compatible with RNA probes biotinylated by different methods

• Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

• Non-radioactive – eliminate waste concerns from radioactive RNA probes