Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Brand: Thermo Scientific™ 20158
715.14 EUR valid until 2025-06-30
Use promo code "24108" to get your promotional price.
Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.Highlights:
Sensitive – chemiluminescent detection comparable to radioactive detection
Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
Flexible – compatible with RNA probes biotinylated by different methods
Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection
Non-radioactive – eliminate waste concerns from radioactive RNA probes
| EMSA Assay | |
| Chemiluminescence | |
| Protein | |
| 100 reactions | |
| LightShift Chemiluminescent RNA EMSA Kit | |
| Not Target-Specific |
| Sufficient For: 100 binding reactions and detection on 1000 cm2 of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C) • 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C) • 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C) • 10 mg/mL tRNA, 100 μL (store at -20°C) • 10X REMSA Binding Buffer, 1 mL (store at -20°C) • 50% Glycerol, 500 μL (store at -20°C) • 2M KCl, 500 μL (store at -20°C) • 1M MgCl2, 500 μL (store at -20°C) • DTT, lyophilized (store at -20°C) • Nuclease-Free Water, 1.5 mL (store at -20°C) • 5X REMSA Loading Buffer, 1 mL (store at -20°C) • Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C) • Luminol/Enhancer Solution, 80 mL (store at 4°C) • Stable Peroxide Solution, 80 mL (store at 4°C) • Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C) • 4X Wash Buffer, 500 mL (store at 4°C) • Substrate Equilibration Buffer, 500 mL (store at 4°C) |
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| Kit | |
| myECL™ Imager, X-Ray Film | |
| LightShift™ | |
| 100 reactions | |
| Enhanced Chemiluminescence, Membrane Blot, Gel Shift |
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For Research Use Only. Not for use in diagnostic procedures.
