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Cytiva MMP-9 Human, Biotrak assay
Provides a simple, specific and precise quantitative determination of MMP-9 in tissue culture supernatants and homogenates as well as plasma
Brand: Cytiva RPN2614
Includes: Anti-MMP-9 Microplate; Assay buffer; Standard; Peroxidase conjugate; Wash buffer; TMB substrate
Description
- Specific for free pro-MMP-9 and pro-MMP-9 complexed to TIMP-1
- No cross-reactivity with pro-MMP-1, -2, -3, TIMP-1 and -2
- Sufficient material for 96 wells. If one standard curve is constructed, 40 unknowns can be measured in duplicate.
- Range: 1-32ng/mL (4–128 ng/ml with plasma)
- Sensitivity: 0.6ng/mL
- Four-hour protocol
Specifications
| MMP-9 | |
| Human | |
| Pipettes or pipetting equipment with disposable polypropylene tips (100 μl, 500 μl, 1 mL and 5 mL); Disposable polypropylene test tubes; Glass measuring cylinders (100 mL and 500 mL); Distilled or deionized water; Spectrophtometric plate reader capable of | |
| Each | |
| 0.6 ng/mL | |
| -15°C to -30°C | |
| Polypropylene |
| 1 to 32ng/mL | |
| MMP-9, Human, Biotrak Assay. | |
| Microplate (12 x 8-well strip coated with Anti-MMP-9), 10mL Phosphate Assay Buffer, 64ng Human pro MMP-9 standard(lyophilized), Anti-MMP-9 HRP Conjugate (lyophilized), 12.5mL Wash Buffer, TMB Substrate | |
| Serum, Plasma (EDTA, Heparin, Citrate), Cell Culture Supernatant | |
| 4 hr. | |
| Suitable for use with serum, plasma (EDTA, heparin, citrate) and cell culture supernatant samplesBiotrak ELISAs measure MMP protein levels in human serum, plasma, and cell culture samples | |
| Human MMP-9 activity assay |