missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. 10074833

Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay
GREENER_CHOICE

Non-radioactive technique

Brand:  Invitrogen™ C10289

421.04 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.

Product Code. 10074833

  • 554.00€ / Each
In Stock
to see stock.
Explore more special offers
Add to basket

Includes: The kit contains sufficient reagents for performing two plates in a 96-well plate format.
This item is not returnable. View return policy

Description

Description

The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne. The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format.

  • Less hazardous

Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Blue, Green
Fluorescence
Fluorescence Microscope, High Content Analysis Instrument
Alexa Fluor™ 488, Hoechst 33258
96-well plate
Alexa Fluor™, Click-iT™, Molecular Probes™
Room Temperature
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
    		•
    High-content Screening (HCS)
    HCS Assay
    UV, Visible
    Less hazardous
    2 plates
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

    For Research Use Only. Not for use in diagnostic procedures.