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Mouse brain tissue lysate, Non-denatured; Abnova

Brand: Abnova L035W2.200ug

Product Code. 16019595

Quantity

Estimated Shipment: 02-01-2025
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Description

Tissue: Brain
Host: Mouse
Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application)	Immunoprecipitation, Western Blot
Description	Whole tissue lysate (non-denatured)
Preparation Method	Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Quantity	200 μg
Host Species	Mouse

Tissue	Brain
Lysis Buffer	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing	12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer	In modified RIPA Lysis Buffer.
Content And Storage	Store at -80°C. Aliquot to avoid repeated freezing and thawing.

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