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Proteintech™ Mouse IL-1 beta ELISA Kit

KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1β ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1β.

Brand:  Proteintech™ KE10003-96T


Product Code. 16861596

  • 269.00€ / 96 tests

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Description

Description

  • The assay recognizes mouse IL-1β. An antibody specific for IL-1β has been pre-coated onto the microwells. The IL-1β protein in samples is captured by the coated antibody after incubation.
  • Following extensive washing, another antibody of biotinylated specific for IL-1β is added to detect the captured IL-1β protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
  • Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation.
  • IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Specifications

Specifications

NP_032387
1 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Mouse
Mouse
<10%
Blue ice
P10749
7.8 to 500 pg/mL
ISO 13485
Coated ELISA Kit
DAB
16176
Solid
<10%
-20°C to +4°C
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