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Novus Biologicals™ pCLNDX Retrovirus Expression Vector
pCLNDX expression vector is part of RetroMax system designed for maximal virus titer in 293 cells
Brand: Novus Biologicals™ NBP2-29539
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Description
The pCLNDX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safety modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.
- RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
- 293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
- Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
- For amplification, transform DH-5alpha or similar bacteria and plate on LB plates containing 50μg/mL ampicillin
- Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Specifications
| 10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA) | |
| Store at -20°C. Avoid freeze-thaw cycles. | |
| 10 μg | |
| pCLNDX Retrovirus Expression Vector |