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Macherey-Nagel Bioanalysis™ NucleoMag 96 PCR Kit with Magnetic Beads

Magnetic bead based PCR clean up for highest flexibility.

Brand:  Macherey-Nagel Bioanalysis™ 744100.4


Product Code. 12783580

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance
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Description

Description

The NucleoMag 96 PCR procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Adjusting the PCR reaction to binding conditions and addition of paramagnetic beads can be carried out simultaneously. After magnetic separation and removal of supernatant, the beads are washed to remove contaminants and salt. A short drying step is necessary to remove ethanol from previous washing steps. Finally, highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The NucleoMag 96 PCR kit can be used either manually or automated on standard liquid handling instruments.

  • Magnetic bead technology
  • Sample Amount: < 50 μL
  • Fragment Size: 150 bp to 10 kbp
  • Typical Recovery: 80 to 95 %
  • Binding Capacity: 0.3 μg/μL beads
  • Preparation Time: 40 to 120 min/96 preps
  • Storage: 18 to 25°C
Specifications

Specifications

PCR Reaction Mixture
25 to 100 μL
PCR clean up
384
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.

Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride

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