Macherey-Nagel Bioanalysis™ NucleoSpin™ 8 Blood QuickPure, 8-Well Kit

Description

Flexible handling of multiples of 8 samples is achieved by use of a reusable column holder. Parallel lysis of large sample numbers can be performed in standard microcentrifuge tubes or in 96 well blocks alternatively. NucleoSpin™ Blood QuickPure binding strips are to be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 8 well strips stacked on 96 well blocks. The method is based on a specially treated silica membrane which speeds up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to only 1, therefore hands-on time is minimised.Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Blood QuickPure binding strips are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR, Southern blotting or any kind of enzymatic reaction. The kit allows isolation of 4 μg to 6 μg of pure genomic DNA from 200 μL whole blood with an A_260/280 ratio between 1.60 and 1.90 and a typical concentration of 40 ng/μL to 60 ng/μL.

Kit components: NucleoSpin™ Blood QuickPure binding strips, buffers, proteinase K, accessories.

• Silica membrane technology
• Manual processing
• Yield: 4 to 6 μg
• Sample material: human or animal whole blood (200 μL/300 μL)
• Elution volume: 7.5 to 100 μL
• Binding capacity: 60 μg
• Preparation time: 60 min/12 strips