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Macherey-Nagel™ NucleoSpin™ 8 Pasmid Kit
Designed for manual or automated purification of high-copy plasmid DNA from E.coli. Macherey-Nagel™ NucleoSpin™ 8 Pasmid Kit is an easy-to-use kit that supplies all core and accessory strips, buffers and RNase A for maximum convenience. The 8-well strip format is ideal for varying throughput.
Brand: Macherey-Nagel™ 740621.5
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Description
NucleoSpin™ 8 Pasmid kits enable variable-throughput isolation of sequencing-grade plasmid DNA from E. coli. The flexible 8-well strip format allows for automation on common liquid handling instruments or for manual processing without time-consuming centrifugation.
Up to 8 samples per strip can be parallel-processed simultaneously, typically within less than 45 minutes. Uses include isolation of high-copy plasmids, purification of cosmids and reliable downstream applications, such as cloning, sequencing, PCR, transformation, and restriction analysis.
- Time-saving parallel isolation of plasmid DNA using silica-membrane technology
- Processing of up to 5mL bacterial culture, with a yield up to 6μg/mL
- 8-Well filter strip for convenient filtration of bacterial lysates
- Suitable for manual or automated processing under vacuum
- Innovative MN Wash Plate minimizes risk of cross-contamination
Specifications
| 8-well Strip | |
| Binding capacity: 20μg | |
| Sequencing grade plasmid DNA | |
| Manual and automated processing under vacuum | |
| 60 x 8 Preps | |
| 1 Kit | |
| 1–5mL | |
| Preparation time: 45 min. / 6 strips |
| E. coli Culture | |
| Elution volume: 75–150μL | |
| Liquid handling instruments | |
| NucleoSpin Plasmid Filter Strips (60), NucleoSpin Plasmid Binding Strips (60), MN Wash Plate (5), Culture plate with Gas-permeable Foil (5), Rack of Tube Strips (5), RNase A (60mg), Resuspension Buffer A1 (150mL), Lysis Buffer A2 (150mL), Neutralization Buffer A3 (200mL), Wash Buffer AW (500mL), Wash Buffer A4 Concentrate (4 x 100mL), Elution Buffer AE (125mL) | |
| NucleoSpin™ 8 Plasmid Kit | |
| A260/A280: 1.70–1.85 | |
| <15kbp | |
| 4–6μg plasmid per 1mL E. coli culture |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt; guanidine hydrochloride; 2-
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