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Macherey-Nagel™ NucleoSpin™ 96 RNA Kit

Designed for manual or automated high throughput isolation of RNA from animal or human tissue. Macherey-Nagel™ NucleoSpin™ 96 RNA Kit enables time-saving parallel isolation of total RNA. The kit supplies all core content and convenient accessories for the highest efficiency.

943.00€ - 9325.00€

Specifications

Concentration	50–200ng/μL
Format	96-well Plate
Isolation Technology	Silica Membrane
Elution Volume	Elution volume: 50–130μL
Sample Type	<1 x 10⁷ Cultured Cells, <30 mg Tissue, Saliva (Collected with Oragene™)

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Product Code	Brand	No. of Reactions	Price	Quantity & Availability
Product Code	Brand	No. of Reactions	Price	Quantity & Availability
12768542 View accessory products View Documents missing translation for 'viewProductCertificates'	Macherey-Nagel™ 740709.24	24 x 96	9325.00€ 2304 reactions	In Stock Log in to see stock.	Add to basket



12736543 View accessory products View Documents missing translation for 'viewProductCertificates'	Macherey-Nagel™ 740709.2	2 x 96	943.00€ 192 reactions	In Stock Log in to see stock.	Add to basket

12756543 View accessory products View Documents missing translation for 'viewProductCertificates'	Macherey-Nagel™ 740709.4	4 x 96	1840.00€ 384 reactions	In Stock Log in to see stock.	Add to basket

Description

NucleoSpin™ 96 RNA kits enable the isolation of RNA from up to 96 samples of fresh, frozen or stabilized animal or human cell cultures. NucleoSpin 96 RNA delivers consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination. The kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by DNase I treatment and the speed of silica-membrane technology.

Time-saving parallel isolation of RNA from cultured cells or from saliva samples collected with Oragene™•RNA
Suitable for manual processing under vacuum or by centrifugation, or used fully automated on common laboratory workstations
Includes rDNase for efficient removal of genomic DNA and innovative MN Wash Plate to minimize the risk of cross-contamination
Produces the highest purity total RNA, suitable for direct use in any kind of enzymatic reaction, such as RT-PCR, TaqMan, Northern Blot or microarray analysis

Kit components: NucleoSpin RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foil, RNase-free rDNase, RNase-free water and all buffers (Lysis RA1; Wash RA2, RA3 and RA4; Reaction for rDNase)

Sample material: <10⁷ cultured cells (centrifugation), <30mg tissue (centrifugation), saliva (collected with Oragene™)
Fragment size: >200 nt
Typical yield: 20μg (2 × 10⁶ HeLa cells), 20μg (20mg mouse liver)
A₂₆₀/A₂₈₀: 1.90–2.10
Typical RIN (RNA integrity number): >9 (cells), ≥7 (tissue)
RNA ratio: 28S/18S approx. 2.1
Typical concentration: 50–200ng/μL
Elution volume: 50–130μL
Preparation time: 70 min/plate
Binding capacity: 100μg

Specifications

Concentration	50–200ng/μL
Isolation Technology	Silica Membrane
Sample Type	<1 x 10⁷ Cultured Cells, <30 mg Tissue, Saliva (Collected with Oragene™)
Product Type	NucleoSpin 96 RNA
Quantity	1 Kit
Yield	20μg (2 × 10⁶ HeLa cells) 20μg (20mg mouse liver)

Format	96-well Plate
Elution Volume	Elution volume: 50–130μL
For Use With (Application)	Manual or automated isolation of total RNA from cultured cells and tissue
Purity	A₂₆₀/A₂₈₀: 1.90–2.10
Test Time	70 min/plate

Safety and Handling

Signal Word

Danger

Hazard Category

Flammable liquid Category 3
Acute toxicity Category 4
Respiratory sensitiser Category 1
LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

H226-Flammable liquid and vapour.
H302-Harmful if swallowed.
H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P264-Wash with water thoroughly after handling.
P273-Avoid release to the environment.
P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
P330-Rinse mouth.
P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

MIXTURE LIST-Contains : guanidinium thiocyanate/ethanol

Macherey-Nagel™ NucleoSpin™ 96 RNA Kit

Specifications

Description

Specifications

Safety and Handling

Documents