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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit
Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.
Brand: Macherey-Nagel™ 740609.240C
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Description
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Specifications
| pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
|
| Silica Membrane Technology | |
| Elution volume: 30–100μL | |
| Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60) | |
| 1 Kit | |
| <400μL (PCR reaction mixture) <400mg (TAE / TBE agarose gel) |
|
| 10 min/6 preps |
| Mini Spin Column | |
| Typical recovery: 70–95% | |
| QIAcube™ compatible TAE or TBE agarose gels, PCR |
|
| 240 Preps | |
| A260/A280: 1.8–1.9 | |
| 50 bp to ∼20 kbp | |
| Up to ∼15μg DNA |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
Supplemental information
- MIXTURE LIST-Contains : guanidinium thiocyanate
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