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  5. Macherey-Nagel™ NucleoSpin™ 8 Pasmid Kit
  6. 12756513

Macherey-Nagel™ NucleoSpin™ 8 Pasmid Kit

Designed for manual or automated purification of high-copy plasmid DNA from E.coli. Macherey-Nagel™ NucleoSpin™ 8 Pasmid Kit is an easy-to-use kit that supplies all core and accessory strips, buffers and RNase A for maximum convenience. The 8-well strip format is ideal for varying throughput.

Brand:  Macherey-Nagel™ 740621


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Product Code. 12756513

  • 326.00€ / 96 reactions
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Description

Description

NucleoSpin™ 8 Pasmid kits enable variable-throughput isolation of sequencing-grade plasmid DNA from E. coli. The flexible 8-well strip format allows for automation on common liquid handling instruments or for manual processing without time-consuming centrifugation.

Up to 8 samples per strip can be parallel-processed simultaneously, typically within less than 45 minutes. Uses include isolation of high-copy plasmids, purification of cosmids and reliable downstream applications, such as cloning, sequencing, PCR, transformation, and restriction analysis.

  • Time-saving parallel isolation of plasmid DNA using silica-membrane technology
  • Processing of up to 5mL bacterial culture, with a yield up to 6μg/mL
  • 8-Well filter strip for convenient filtration of bacterial lysates
  • Suitable for manual or automated processing under vacuum
  • Innovative MN Wash Plate minimizes risk of cross-contamination
Specifications

Specifications

8-well Strip
Binding capacity: 20μg
Sequencing grade plasmid DNA
Manual and automated processing under vacuum
12 x 8 Preps
1 Kit
1–5mL
Preparation time: 45 min. / 6 strips
E. coli Culture
Elution volume: 75–150μL
Liquid handling instruments
NucleoSpin Plasmid Filter Strips (12), NucleoSpin Plasmid Binding Strips (12), MN Wash Plate (1), Culture plate with Gas-permeable Foil (1), Rack of Tube Strips (1), RNase A (30mg), Resuspension Buffer A1 (75mL), Lysis Buffer A2 (75mL), Neutralization Buffer A3 (100mL), Wash Buffer AW (100mL), Wash Buffer A4 Concentrate (100mL), Elution Buffer AE (30mL)
NucleoSpin™ 8 Plasmid Kit
A260/A280: 1.70–1.85
<15kbp
4–6μg plasmid per 1mL E. coli culture
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.

Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt; guanidine hydrochloride; 2-

SDS
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