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Macherey-Nagel™ NucleoSpin™ RNA Columns

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

Brand: Macherey-Nagel™ 740955.50S

Product Code. 15880968

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Description

NucleoSpin™ RNA

rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
Up to 70 μg ready to use RNA
Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
RNA isolation from cultured cells, tissue (standard protocol):
- RNA from < 10⁹ bacterial cells (Gram-negative, Gram-positive) or < 10⁸ yeast cells
- RNA from ≤ 100 μL biological fluids
- RNA clean-up from reaction mixtures
- RNA from samples stored in RNAlater™
- RNA from saliva samples collected with Oragene™ RNA (Genotek)
Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

Color	Blue band
Format	Mini Spin Column
Elution Volume	40 - 120 μL
Efficiency	Binding capacity: 200 μg
For Use With (Application)	Isolation of RNA from cells and tissue
Quantity	50 Columns
Test Time	35 min/6 preps

Content And Storage	rDNase at 4 °C; Other components at room temperature (18 - 25 °C)
Isolation Technology	Silica Membrane
Sample Type	Cultured Bacterial or Yeast Cells, Human or Animal Tissue
For Use With (Equipment)	NucleoSpin RNA Kits
Includes	Columns only
Sample Size	< 30 mg tissue, 5 x 10⁶ cultured cells

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Supplemental information

MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu