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Tocris Bioscience™ PA Janelia Fluor 549, SE
Yellow photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
Brand: Tocris Bioscience™ 6149/300U
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Description
Key information: PA Janelia Fluor™ 549, SE is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging. Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques. Properties and Photophysical Data: Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Can be multiplexed with PA Janelia Fluor™ 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively. Please see the protocol for further information on protein/antibody labeling and conjugation. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.Specifications
| 2,5-Dioxo-1-pyrrolidinyl 3,6-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9-[9H]xanthene]-6-carboxylate | |
| 300 μg | |
| C32H25N5O6 |
| 1811539-42-0 | |
| Photoactivatable Dyes | |
| 90% |
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