missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. High Fidelity PCR Reagents and Kits
  7. Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
  8. 10287787

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Brand:  Thermo Scientific™ F530L

357.50 EUR valid until 2025-06-30
Use promo code "24108" to get your promotional price.

 View more versions of this product

Product Code. 10287787

  • 550.00€ / 500 units

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
Phusion High-Fidelity DNA Polymerase
High-Fidelity DNA Polymerase
Dry Ice
2 U/μL
No
Blunt
20 kb or less
High
Fast
500 units
Standard PCR, High-fidelity PCR
52X
500 Reactions
Standalone
Colorless
Product Suggestions

Product Suggestions

Videos
SDS
Documents Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number3113396 Certificate TypeCertificate of Analysis Date21/02/2025 Catalog NumberF530L
Lot Number3123988 Certificate TypeCertificate of Analysis Date21/02/2025 Catalog NumberF530L
Lot Number3099304 Certificate TypeCertificate of Analysis Date21/02/2025 Catalog NumberF530L
Lot Number3113631 Certificate TypeCertificate of Analysis Date21/02/2025 Catalog NumberF530L
Lot Number3152115 Certificate TypeCertificate of Analysis Date21/02/2025 Catalog NumberF530L
Special Offers

Special Offers

  • Invitrogen , ABI Applied Biosystems , Thermo Scientific , Affymetrix eBioscience

    Savings to support your research.
    Promo Code: 24108

    Expires:  2025-05-27

    New Offer
    Save Now
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL) > Colorless, 500 Reactions

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.