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Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

191.00€ - 832.00€

Specifications

GC-Rich PCR Performance High
Polymerase Phusion U Hot Start DNA Polymerase
Reaction Speed Fast
Product Type Hot Start DNA Polymerase
Shipping Condition Dry Ice
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Products 4
Product Code Brand Color No. of Reactions Price Quantity & Availability  
Product Code Brand Color No. of Reactions Price Quantity & Availability  
15250143
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Thermo Scientific™
F556L
Green 500 Reactions
832.00€
500 units
In Stock
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15240143
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Thermo Scientific™
F556S
Green 100 Reactions
192.00€
100 units
Estimated Shipment: 21-01-2025
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15230143
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Thermo Scientific™
F555L
Colorless 500 Reactions
828.00€
500 units
In Stock
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15220143
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Thermo Scientific™
F555S
Colorless 100 Reactions
191.00€
100 units
In Stock
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Description

Description

Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Highlights

  • Accuracy—high fidelity DNA polymerase (25X Taq)
  • Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
  • Specificity—hot start for reduced nonspecific amplification and primer degradation
  • Speed—short extension times (15–30 s/kb)
  • Green format—permits direct loading of PCR products on gels

Applications

  • Amplification of bisulphite-converted DNA
  • Amplification of damaged or aged DNA
  • Carry-over contamination control
  • Uracil-excision based (USER) cloning methods

Using Phusion DNA polymerases

Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

Specifications

High
Fast
Dry Ice
2 U/μL
Built-In Hot Start
Standalone
Phusion U Hot Start DNA Polymerase
Hot Start DNA Polymerase
Hot-start PCR, High-fidelity PCR
25X
Blunt
20 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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