Invitrogen™ Pierce™ Avidin, Fluorescein (FITC) Conjugated

Description

Thermo Scientific™ Pierce Avidin and Conjugates include purified avidin protein from hen egg whites, plus avidin-HRP, avidin-AP and other conjugated forms for use in biotin-based detection methods.

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

• Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

• Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

• Affordable – significantly less expensive than recombinant streptavidin

• ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

• Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays