Thermo Scientific™ IPTG

Description

Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate forβ-Galactosidase. It yields a blue precipitate, is soluble in dimethylformamide, and can be used to detect vectors, plasmids or DNA fragments that contain the β-Galactosidase gene.

Highlights:

• See item-specific features

X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside)

• Chromogenic substrate for β-Galactosidase
• Yields a blue precipitate
• Soluble in DMF
• Can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene

IPTG (Isopropyl-β-D-thiogalactopyranoside)

• Maximizes the expression of genes in expression vectors
• When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established
• By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after three to four hours of plaque growth; the nitrocellulose filter is then screened with antibodies
• IPTG is also used in combination with X-Gal to detect active β-galactosidase
• Practical in applications using Bacteriophage M13 and Igt11 vectors