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Thermo Scientific™ Pierce™ Biotin-Fluorescein Conjugate

Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).

Brand:  Thermo Scientific™ 22030

335.92 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


Product Code. 10752905

  • 442.00€ / 5mg
Estimated Shipment: 17-12-2024
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Description

Description

Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Highlights:

  • Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining

  • Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

  • Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Classic Dyes, Biotin and Analogs
5 mg
494/518
Solid
Biotin Conjugate (Fluorescein)
Pierce Biotin-Fluorescein Conjugate
Store at 4°C, protected from moisture. Shipped at room temperature.
DMF (Dimethylformamide)
Pierce™
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Safety and Handling

Safety and Handling

Product Identifier
  • Biotin-Fluorescein

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information
  • MIXTURE LIST-Contains : Biotin-Fluorescein

SDS
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.