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Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Brand: Thermo Scientific™ 34000
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Description
Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.
This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.Highlights:
HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph
Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve
Stable – store refrigerated at 4°C for at least one year
Includes:
10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer
Specifications
| Substrate | |
| Liquid | |
| 275 mL | |
| Horseradish Peroxidase | |
| 25 Uses at 10 mL Each |
| Colorimetric | |
| Pierce™ | |
| HRP | |
| Horseradish Peroxidase | |
| Sufficient For: 25 uses at 10 mL each • CN/DAB (10X), 25 mL • Stable Peroxide Buffer, 250 mL Store at 4°C. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- CN/DAB Substrate Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Flammable liquid Category 2
- Specific target organ toxicity after single exposure Category 1
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : methanol
For Research Use Only. Not for use in diagnostic procedures.