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Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

Biotinylate single-stranded DNA primers for non-radioactive EMSA and other nucleic acid detection methods.

Brand: Thermo Scientific™ 89818

562.40 EUR valid until 2025-12-31
Use promo code "24344" to get your promotional price.

Product Code. 10036813

654.00€ / 1 set

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Description

Thermo Scientific™ Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency. The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations, or in situ hybridizations.

Highlights:

Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
Biotin-labeled probes are stable for more than one year
30-minute labeling procedure is fast and efficient

Includes:

1mL 5X TdT Reaction Buffer, 50μL Terminal Deoxynucleotidyl Transferase, 100microliter Biotin-11-dUTP, 140μL Unlabeled Control Oligo, 40μL Biotin-Control Oligo

Specifications

Content And Storage	Sufficient For: 20 labeling reactions (each with 5 pmol DNA) • 1 mL 5X TdT Reaction Buffer (-20°C) • 5 μL Terminal Deoxynucleotidyl Tranferase (-20°C) • 1 μL Biotin-11-UTP (≤4°C) • 14 μL Unlabeled Control Oligo (-20°C) • 4 μL Biotin-Control Oligo (-20°C)
Labeling Target	DNA
Product Line	Pierce™
Quantity	20 Reactions

Format	Kit
No. of Reactions	20
Product Type	DNA Labeling Kit

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Pierce Biotin 3' End DNA Labeling Kit

Signal Word

Danger

Hazard Category

Acute toxicity Category 4
LONG-TERM AQUATIC HAZARD Chronic 2
Carcinogenicity Category 1B

Hazard Statement

H302-Harmful if swallowed.
H350-May cause cancer.
H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

P201-Obtain special instructions before use.
P273-Avoid release to the environment.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
P308+P313-IF exposed or concerned: Get medical advice/attention.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Cobalt(II) chloride,hexahydrate

Documents

Scientific Resources

Application Notes

Tech Tip: Anneal complementary pairs of oligonucleotides File size: 158.6 kB

Product Information

Manuals

User Guide: Biotin 3ft End DNA Labeling Kit Version: Jan. 2015 File size: 118.1 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZF391991	Certificate TypeCertificate of Analysis	Date10/07/2024	Catalog Number89818
Lot NumberZC375713	Certificate TypeCertificate of Analysis	Date09/03/2024	Catalog Number89818
Lot NumberYJ381940	Certificate TypeCertificate of Analysis	Date18/10/2023	Catalog Number89818
Lot NumberYE372382	Certificate TypeCertificate of Analysis	Date24/05/2023	Catalog Number89818
Lot NumberYA369873	Certificate TypeCertificate of Analysis	Date10/02/2023	Catalog Number89818

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Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit > 3' DNA end Biotinylation Kit

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