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Thermo Scientific™ Fluorescein-5-Maleimide
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Brand: Thermo Scientific™ 62245
This item is not returnable.
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Description
Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.Highlights:
- Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
Spezifikation
| 25 mg | |
| DMF (Dimethylformamide) | |
| Thiol | |
| FITC (Fluorescein) | |
| Pierce™ | |
| 494 |
| Maleimide | |
| Store desiccated at -20°C, protect from moisture | |
| Maleimide | |
| Classic Dyes | |
| 518 |
Sicherheit und Handhabung
Produktidentifikator
Signalwort
Gefahrenkategorie
Gefahrenhinweis
Sicherheitshinweis
Ergänzende Informationen
- Fluorescein-5-Maleimide, 25 mg
Signalwort
- Warning
Gefahrenkategorie
- Acute toxicity Category 4
Gefahrenhinweis
- H302-Harmful if swallowed.
Sicherheitshinweis
- P270-Do not eat, drink or smoke when using this product.
- P264-Wash thoroughly after handling.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- P501-Dispose of contents/container to .
Ergänzende Informationen
- MIXTURE LIST-Contains : Fluorescein-5-maleimide
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Name des Produkts
Thermo Scientific™ Fluorescein-5-Maleimide >
For Research Use Only. Not for use in diagnostic procedures.
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