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Thermo Scientific™ FITC (5/6-fluorescein isothiocyanate), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Brand: Thermo Scientific™ 46425
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Description
Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.Highlights:
- Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
Specifications
| 100 mg | |
| DMF (Dimethylformamide) | |
| Amine | |
| FITC (Fluorescein) | |
| Pierce™ | |
| 494 |
| FITC | |
| Store desiccated at -20°C, protect from moisture | |
| Isothiocyanate | |
| Classic Dyes | |
| 518 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- FITC (5/6-fluorescein isothiocyanate), mixed isomer
Signal Word
- Danger
Hazard Category
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P310-Immediately call a POISON CENTER/doctor/.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl) -5-isothiocyanatobenzoic acid
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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