Thermo Scientific™ NeutrAvidin Protein, AP

Description

NeutrAvidin Protein, AP conjugate, 31002, from Invitrogen., 31002, from Invitrogen. Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.

NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14µg/mg of protein, which is near the theoretical maximum activity.
Formulation: 2mg purified protein (2-4mg/mL) in TBS with 50% glycerol, 0.1mMmgCl2, 0.1mM ZnCl2 and no preservative; pH 8
This product has been successfully used in ELISA, Immunohistochemistry, In Situ Hybridization (ISH) and Western Blot procedures.