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Thermo Scientific™ Pierce™ Protein Coated Plates
Thermo Scientific™ Pierce Protein A, G, A/G and L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.
247.00€ - 328.00€
Specifications
Binding Property | Standard Capacity, Affinity |
---|---|
Product Line | Pierce |
Material | Polystyrene |
No. of Wells | 96 |
No. per Pack | 5 Plates |
Product Code | Brand | Surface Treatment | Description | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Surface Treatment | Description | Price | Quantity & Availability | ||||
10248343
|
Thermo Scientific™
15132 |
Protein A | Clear, 8-well Strip |
300.00€
Pack of 5 |
Estimated Shipment: 15-01-2025
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10495005
|
Thermo Scientific™
15130 |
Protein A | Clear, 96-well |
247.00€
Pack of 5 |
In Stock
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||||
10298153
|
Thermo Scientific™
15155 |
Protein A | Black, 96-well |
301.00€
Pack of 5 |
Estimated Shipment: 15-01-2025
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||||
10094453
|
Thermo Scientific™
15154 |
Protein A | White, 96-well |
302.00€
Pack of 5 |
Estimated Shipment: 21-01-2025
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||||
10063883
|
Thermo Scientific™
15133 |
Protein G | Clear, 8-well Strip |
322.00€
Pack of 5 |
In Stock
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||||
10405015
|
Thermo Scientific™
15131 |
Protein G | Clear, 96-well |
301.00€
Pack of 5 |
Estimated Shipment: 15-01-2025
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||||
10043933
|
Thermo Scientific™
15157 |
Protein G | Black, 96-well |
328.00€
Pack of 5 |
Estimated Shipment: 15-01-2025
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||||
10338733
|
Thermo Scientific™
15156 |
Protein G | White, 96-well |
328.00€
Pack of 5 |
Estimated Shipment: 15-01-2025
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||||
10073883
|
Thermo Scientific™
15138 |
Protein A/G | Clear, 8-well Strip |
328.00€
Pack of 5 |
Estimated Shipment: 21-01-2025
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||||
10658534
|
Thermo Scientific™
15190 |
Protein L | Clear, 96-well |
317.00€
Pack of 5 |
Estimated Shipment: 10-01-2025
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Description
These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.
Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.
Highlights
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)
Protein A/G Coated Plates
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH
Protein A Coated Plates
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation
Protein G Coated Plates
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation
Protein L Coated Plates
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding
Specifications
Standard Capacity, Affinity | |
Polystyrene | |
5 Plates |
Pierce | |
96 | |
SuperBlock™ Buffer |
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