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Thermo Scientific™ Pierce™ Recombinant Protein G

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Brand:  Thermo Scientific™ 77676

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Product Code. 15295683

  • 2715.00€ / 500mg

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Description

Description

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA
TRUSTED_SUSTAINABILITY
Specifications

Specifications

500 mg
Powder
Antibodies
Pierce™
Protein G
Upon receipt store at -20°C. Product is shipped at ambient temperature.
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZF385005 Certificate TypeCertificate of Analysis Date26/06/2024 Catalog Number77676
Lot NumberYJ383039 Certificate TypeCertificate of Analysis Date24/10/2023 Catalog Number77676
Lot NumberYH382528 Certificate TypeCertificate of Analysis Date23/08/2023 Catalog Number77676
Lot NumberYG378244 Certificate TypeCertificate of Analysis Date22/07/2023 Catalog Number77676
Lot NumberYD377260 Certificate TypeCertificate of Analysis Date03/05/2023 Catalog Number77676
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Thermo Scientific™ Pierce™ Recombinant Protein G > 500mg

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For Research Use Only. Not for use in diagnostic procedures.