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Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit
Biotinylate 3-prime terminal ends of RNA probes for non-radioactive EMSA and other nucleic acid detection methods.
Brand: Thermo Scientific™ 20160
699.20 EUR valid until 2024-12-31
Use promo code "21954" to get your promotional price.
This item is not returnable.
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Description
Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.
The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents. The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency.Highlights:
Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems
Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing
Easy to use – RNA ligase and optimized reaction buffer are included
Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes
End-labeled – results in minimal disturbance of RNA secondary structure
Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides
Specifications
| Sufficient For: 20 labeling reactions (each with 50 pmol RNA) • T4 RNA Ligase Reaction Buffer (10X), 100 μL • T4 RNA Ligase (20,000 U/mL), 40 μL • Non-labeled RNA Control (10 μM), 100 μL • Biotinylated IRE RNA Control (125 nM), 35 μL • RNase Inhibitor (40 U/μL), 2 × 10 μL • Nuclease-free Water, 1.5 mL • DMSO, 200 μL • 30% PEG, 300 μL • Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL • Glycogen (20 mg/mL), 20 μL Store all components at -20°C |
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| Pierce™ | |
| 20 Reactions |
| Kit | |
| RNA Labeling Kit |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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